liqun luo (Addgene inc)

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Addgene inc liqun luo
Liqun Luo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/liqun+luo/pUAS-luc2+(Plasmid+%2324343)/pmc12915196-366-12-14
Average 94 stars, based on 19 article reviews

liqun luo - by Bioz Stars, 2026-10

94/100 stars

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Plasmid Preparation:

Article Title: Calcium and Cadmium Activate ESRRB to Mediate Cell Stemness and Pluripotency
Article Snippet: The plasmid for ESRRB variant 2 (pSG5-ERRbeta2) was a gift from Dr. Rebecca Riggins, Georgetown University (Addgene plasmid #52186; RRID: Addgene 52186; Addgene, Watertown, MA, USA) [ ]. .. The plasmid containing GAL4 (pCMV-GAL4) was a gift from Liqun Luo (Addgene plasmid #24345; RRID:Addgene_24345; Addgene, Watertown, MA, USA) [ ]. .. The β-galactosidease (pEQ176) plasmid was a gift from Adam Geballe (Addgene plasmid #83943; RRID:Addgene_83943; Addgene, Watertown, MA, USA) [ ] and the GAL4-CAT reporter plasmid pG6(5′Pro) (P#1477) containing the chloroamphenicol acetyltransferase gene was a gift from Matija Peterlin (Addgene plasmid #14659; RRID:Addgene_14659; Addgene, Watertown, MA, USA) [ ].

Article Title: The serotonin receptor 2b (5-HT2B) modulates heart remodelling following myocardial infarction via regulation of Hippo pathway
Article Snippet: The Ad-GAL4-TEAD was generated from GAL4-TEAD plasmid, which was a gift from Kunliang Guan (Addgene plasmid # 24640; http://n2t.net/addgene:24640 ; RRID:Addgene_24640). .. The Ad-UAS-luciferase was generated from pUAS-luc2, which was was a gift from Liqun Luo (Addgene plasmid # 24343 ; http://n2t.net/addgene:24343 ; RRID:Addgene_24343). .. NIH/3T3 fibroblasts (ATCC) were stored at -196°C in liquid nitrogen prior to rapid thawing in a 37°C waterbath for 2 min. 1 mL thawed cell solution was diluted with 9 mL culture media (90% DMEM, 10% FBS and 100 units/mL penicillin and streptomycin) and centrifuged at 1000 rpm for 5 min.

Article Title: A nanobody toolbox for recognizing distinct epitopes on Cas9.
Article Snippet: .. The dose-response to a Cas9 ribonucleoprotein complex (RNP) was determined as above except using an RNP formed from Cas9 and a sgRNA targeted to the ROSA mT/mG plasmid, a gift from Liqun Luo (Addgene #17787) [20]. ..

Article Title: Neuropeptide Y neurons in the basolateral amygdala project to the nucleus accumbens and stimulate high-fat intake
Article Snippet: .. pAAV-EF1a-DIO-mCherry (Addgene plasmid #50462; RRID:Addgene_50462 ) was a gift from Bryan Roth. rAAV2-retro helper (Addgene plasmid #81070; RRID:Addgene_81070 ) was a gift from Alla Karpova and David Schaffer ( ). pAAV-hSyn-FLEx-mGFP-2A-Synaptophysin-mRuby (Addgene plasmid #71760; RRID:Addgene_71760 ) was a gift from Liqun Luo ( ). pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (Addgene plasmid #20298; RRID:Addgene_20298 ) and pAAV-Ef1a-DIO -eNpHR3.0-EYFP (Addgene plasmid #26966; RRID:Addgene_26966 ) were gifts from Karl Deisseroth ( ). .. For recombinant AAV (rAAV) production, HEK293 cells were cotransfected with the vectors pAAV-DJ (Cell Biolabs, San Diego, CA, USA) or rAAV2-retro helper and pHelper (Takara Bio Inc., Shiga, Japan) using the AAVpro Helper Free system (Takara Bio Inc.). rAAV particles were extracted and purified using the AAVpro Purification Kit (Takara Bio Inc.), according to the manufacturer’s instructions. rAAV titers were determined by quantitative PCR using the AAVpro Titration Kit (Takara Bio Inc.).

Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Article Snippet: .. Gal4-TEAD4 was a gift from Kunliang Guan (Addgene plasmid #24640; http://n2t.net/ addgene:24640; RRID:Addgene_24640) and pUAS-luc2 was a gift from Liqun Luo (Addgene plasmid #24343; http://n2t.net/addgene:24343; RRID:Addgene_24343) [29]. .. The constructs were cloned to adenovirus vector pAd-CMV-DEST (Invitrogen, Life Technologies, Warrington, UK) using a Gateway cloning system. iPSC-CMs were transduced with AdGal4-TEAD/UAS for 24 h, and then induced with YAPS127A or treated with XMU-MP-1 for a further 24 h before assessing YAP activity using a Luciferase Assay System (Promega, Southampton, UK).

Article Title: The serotonin receptor 2b (5-HT 2B ) modulates heart remodeling following myocardial infarction via regulation of Hippo pathway
Article Snippet: The Ad-GAL4-TEAD was generated from GAL4-TEAD plasmid, which was a gift from Kunliang Guan (Addgene plasmid # 24640; http://n2t.net/addgene:24640 ; RRID:Addgene_24640). .. The Ad-UAS-luciferase was generated from pUAS-luc2, which was was a gift from Liqun Luo (Addgene plasmid # 24343; http://n2t.net/addgene:24343 ; RRID:Addgene_24343). .. For YAP localisation experiments, cells were infected with Ad-GFP-YAP, generated from the plasmid construct pEGFP-C3-hYAP1, which was a gift from Marius Sudol (Addgene plasmid # 17843; http://n2t.net/addgene:17843 ; RRID:Addgene_17843).

Article Title: A Nanobody Toolbox for Recognizing Distinct Epitopes on Cas9
Article Snippet: .. The dose–response to a Cas9 ribonucleoprotein complex (RNP) was determined as above except using an RNP formed from Cas9 and a sgRNA targeted to the ROSA mT/mG plasmid, a gift from Liqun Luo (Addgene #17787). ..

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Article Title: Molecular characterization of nervous system organization in the hemichordate acorn worm Saccoglossus kowalevskii
Article Snippet: Injection mixtures contained 10μl restriction digest including 5 units 1097 of I-SceI enzyme (NEB), 1μl CutSmart buffer, and 130ng of reporter plasmid, final concentration 1098 13ng/μl.

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